Figure 7: Proliferation Activity in the Pulmonary Vasculature

Representative images for Ki67 immunofluorescence staining. (A) Examples from the MCT animal model. (B) Examples from the SuHx model. Ki67 (white), α-smooth muscle actin (red), nuclei (DAPI) (scale bar, 20 μm). (C and D) RD reduced the proliferation in the pulmonary vasculature. Control: n = 4, MCT-sham: n = 5, and MCT-RD: n = 4. Control: n = 4, SuHx-sham: n = 4, and SuHx-RD: n = 4. Data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni correction. Ki67 = cell proliferation marker; other abbreviations as in Figures 1 and 6.