Figure 6: GLUT4 Is a SUR2-Interacting Protein

(A) Quantitative PCR analysis of cDNA of whole hearts from tamoxifen-treated Cre– and Cre+ showed similar transcript levels of Slc2a4, which encodes glucose transporter 4 (GLUT4), and Slc2a1, which encodes GLUT1 (n = 7 per group). (B) Cre– and Cre+ hearts express similar levels of GLUT4 and GLUT1 protein (n = 7 per group), as measured by quantitative immunoblotting. (C) SUR2, SUR55, and KIR6.2 each co-immunoprecipitated with GLUT4 when co-transfected in human embryonic kidney cells (arrows). Immunoprecipitation with immunoglobulin G (IgG) and beads alone did not result in co-immunoprecipitation and are shown as controls. (D) Full-length SUR2 is co-immunoprecipitated with GLUT4 from normal hearts, indicating this interaction occurs with native proteins. The interaction between GLUT4 and SUR2 was not altered in the presence of insulin. Immunoprecipitation (IP) with IgG is shown as a control. IB = immunoblot; GFP = green fluorescent protein; other abbreviations as in Figures 1 and 2.



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