Figure 6: Effects of TSG-6 on Inflammatory Phenotype and Marker Expression in Peritoneal Exudate Macrophages From ApoE

Peritoneal cells obtained from ApoE−/− mice were suspended in conditioned medium and seeded onto 6-cm dishes (4 × 106 cells per 2 ml/dish). After incubation for 1 h at 37°C in 5% CO2 to allow adhesion, the medium was discarded to remove nonadherent cells. Immediately, adherent macrophages were harvested after washing the dishes with phosphate-buffered saline twice for immunoblotting analyses of MARCO (M1 marker), arginase-1 (M2 marker), MCP-1, pentratin-3, COX-2, NF-κB, JNK, and phosphorylated ERK1/2. β-actin served as a loading control. n = 3 to 5; *p = 0.018; †p = 0.031; ‡p = 0.012; #p = 0.041; §p = 0.038; ¶p < 0.0001 vs. control. All values are presented as mean ± SEM. Abbreviations as in Figures 3 and 5.