Figure 6: Angiotensin II Type 1 Receptor and Mineralocorticoid Density in the Pulmonary Vasculature

Representative images for AT1-receptor and MR-receptor immunofluorescence staining. (A) Examples from the MCT animal model. (B) Examples from the SuHx model. AT1-receptor (red), smooth muscle cells (SM22) (green), and nuclei (DAPI) (scale bar, 20 μm). (C and D) RD reduced AT1-receptor density in the pulmonary vasculature. (E) Examples from the MCT animal model. (F) Examples from the SuHx model: MR-receptor (red), SM22 (green), and nuclei (DAPI) (scale bar, 20 μm). (G and H) RD reduced MR-receptor density in the pulmonary vasculature in the SuHx-model. Control: n = 4, MCT-sham: n = 4, and MCT-RD: n = 4. Control: n = 4, SuHx-sham: n = 4, and SuHx-RD: n = 4. Data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni correction. AT1= angiotensin II type 1 receptor; DAPI = 4′,6-diamidino-2-phenylindole; MR = mineralocorticoid receptor; SM = smooth muscle; other abbreviations as in Figure 1.