Figure 5: The N-terminally Truncated β

2019-06-11T07:09:18Z (GMT) by Misun Park Susan F. Steinberg
(A) Cardiomyocytes that heterologously overexpress similar levels of FL or N-terminally truncated β1ARs (Δ2-52) (as validated according to both immunoblot analysis and radioligand binding with 125I-iodocyanopindolol, based on protocols detailed elsewhere [12]) were treated for 24 h with vehicle or doxorubicin. Lysates were probed for β1AR expression, AKT phosphorylation, and caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavage, with β-actin immunoreactivity as a loading control. A representative experiment is depicted on the left; results for pAKT (n = 9), caspase-3 cleavage (n = 4), or PARP cleavage (n = 6) are quantified on the right (with all results normalized to immunoreactivity in corresponding vehicle-treated FL-β1AR expressing cardiomyocytes). (B) Effects of PTX (100 ng/ml) on AKT phosphorylation in FL-β1AR or Δ2-52-β1AR overexpressing cardiomyocytes. The data are representative of results obtained in 3 separate culture preparations. Abbreviations as in Figures 1, 2, 3, and 4.



Elsevier user license