Figure 5: TT-10 Ameliorates Cardiac Dysfunction After MI in Mice

(A) Echocardiographic analysis after myocardial infarction (MI). Representative images are shown. n = 23 to 24 (vehicle) and n = 22 to 23 (TT-10). (B) Fractional areas of infarction and fibrosis as determined by Picrosirius Red/Fast Green staining and representative cross sections of the hearts from 2 mice (#1 to 2) per group. n = 8 (vehicle) and n = 11 (TT-10). Scale bar: 1 mm. (C and D) Quantitative analyses of pH3-positive CMs (C) and Aurora B-positive CMs (D) 1 week after MI. Representative images of the infarct border-zone myocardium are shown. Inset images were enlarged by z-stack confocal microscopy. Arrowheads indicate positive CMs. White asterisks indicate infarct area. n = 6 per group. Scale bars in C and D: 50 μm (25 μm in insets). (E) Nuclear YAP expression in infarct border-zone CMs with representative images. Nuclear YAP expression was not detected in sham operated mice heart. CMs restored YAP activation after MI, and TT-10 treatment further enhanced the numbers of such CMs. ND = not detected. White asterisks indicate infarct area. n = 3 (MI [−]), 4 (vehicle), and 6 (TT-10). Scale bars: 100 μm (25 μm in inset). *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle control. AWth = anterior wall thickness; EF = ejection fraction; LVDd = left ventricular end-diastolic dimensions; LVDs = left ventricular end-systolic dimension; PWth = posterior wall thickness; other abbreviations as in Figures 1 and 2.