Figure 5: Rad

(A) Representative images of sequential short-axis heart sections from hearts subjected to global no-flow ischemia stained with 0.1% triphenyltetrazolium chloride (TTC) revealing viable (red) and nonviable (white or beige) myocardium. (B) Quantification of infarct volume as a percentage of heart volume shows no difference in infarct development between wild-type (WT) and Rad−/− hearts. N = 8 mice per group. (C) Representative images of heart sections 24 h after LAD ligation stained with Evans blue/TTC. Blue area is perfused tissue, red area indicates living nonperfused tissue, and white/beige area is infarcted (nonviable) tissue. (D,E) Quantification of area at risk (AAR) and infarct reveals no difference in nonperfused tissue area or infarct development between WT and Rad−/− hearts. (F) Gadolinium magnetic resonance imaging of infarcted hearts also reveals comparable infarction at 24 h between WT and Rad−/− mice. (G) No difference is observed in 24-h apoptosis staining. White arrows show apoptotic cells. N = 8 mice per group.