Figure 5: Immunoblotting Results for 3-NT

These demonstrate a significant increase in the 47-kDa band (p = 0.003) and a borderline increase in the 70-kDa band (p = 0.05) (2-way ANOVA) with ISO treatment without statistically significant regional variability between apex and base. Five control rats were compared with 5 ISO-treated rats, with blots completed in triplicate. Western blots; CA = control apex; CB = control base; IA = ISO apex; IB = ISO base; other abbreviations as in Figure 3.