Figure 5: Histopathologic Differences Between Groups

Regions of radiographically apparent injury (A) (arrows) correlated well with histologically apparent injury (B), shown here by fluorescence microscopy (original magnification: ×1, using a Rhodamine filter). Neuronal injury was scored between 0 (normal) and 5 (severe neuronal injury, necrosis) for each region through identification of hypereosinophilic and/or apoptotic neurons by using both light (C) and fluorescence (D) microscopy (original magnification: ×60; bars = 50 μm). Open arrows = hypereosinophilic and apoptotic neurons. (E) As a group, hydrogen-treated swine exhibited significantly lower histologic injury scores than controls (2-way repeated measures ANOVA according to Sidak's test results: p = 0.0044). Data are based on histopathology for 8 swine in the hydrogen-treated group and 6 in the control group (2 swine allocated to the control group died from refractory seizures and did not survive to day 3). Data are means; error bars are SEM.