Figure 5: Effects of AV-R1 + AV-R2 Treatment on ARCs

(A) Contractile response of adult rat cardiomyocytes (ARCs) at different stimulation frequencies. AV-R1 + AV-R2–treated cells (triangles) showed significantly greater response to Ca2+ at all frequencies. AV-GFP–treated cells (open circles); nontransduced cells (solid circles). *p < 0.05 compared with nontransduced; †p < 0.05 compared with AV-GFP–treated. (B to D) R1R2 protein expression in ARCs after AV-R1 + AV-R2 treatment. Increased R1 (B) and R2 (C) protein expression in AV-R1 + AV-R2–treated neonatal rat ventricular myocytes (NRVMs). (D) Increased intracellular dATP in AV-R1 + AV-R2–treated NRVMs. *p < 0.05 compared with AV-GFP–treated NRVMs. GFP = green fluorescent protein.