Figure 4: Upregulated Genes in LDGs Show Increased Binding to Platelets

RNA sequencing analysis shows an (A) upregulation of platelet-specific biological pathways in psoriasis LDGs versus psoriasis NDGs. Significance was established by FDR. (B) Neutrophil platelet aggregates were increased in psoriasis patients (n = 12) compared with matched control subjects (n = 10). Data are represented as mean ± SEM. Significance was established by the unpaired Mann-Whitney Student’s t-test and set at *p < 0.05. (C) The platelet-specific transcriptomes were upregulated in LDGs compared with NDGs (n = 7). (D) The platelet receptor, CD36, was highly upregulated in LDGs, and the FPKM values were associated with (E) NCB (n = 7). (F) Flow cytometry plots show (G) aggregates of NDGs or LDGs with platelets and the percentages of platelets LDG aggregates are (H) highly associated with NCB. (I) Scanning electron microscopy images of NDGs and LDGs demonstrate platelet LDG aggregates. Scale bar: 10 μm. CD40LG = CD40 ligand; F2R = coagulation factor II thrombin receptor; GP9 = glycoprotein IX platelet; ITGB3 = integrin subunit beta 3; PF4 = platelet factor 4; PPBP = pro-platelet basic protein; SELP = selectin P; SELPLG = selectin P ligand; TREML1 = triggering receptor expressed on myeloid cells like 1; TUBB1 = tubulin beta 1 class VI; other abbreviations as in Figures 1, 2, and 3.