Figure 4: SEM Images of Sinuses After Sterilization by Various Techniques

(A) Nonsterile decellularized sinuses showed the presence of microbes (arrows). (B) Sterilization with the EOW technique could not remove the microbes (arrows). (C) Microbes (arrows) were evident after sterilization with gamma radiation. (D) No microbes were found after sterilization with the EPTA technique. (E) Presence of microbes (arrows) proved that the LHP technique could not sterilize sinus tissue completely. (F) Sinus tissues were completely sterile because no microbes were present after sterilization with the scCO2 technique. Scale = 12 μm. Abbreviations as in Figures 2 and 3.