Figure 4: Oxidative Adducts in Chagasic Myocardium (±SIL)

Mice were infected with T. cruzi and treated with SIL as described in the text. (A) Frozen heart tissue sections were incubated with MitoSOX Red (detects mitochondrial reactive oxygen species, fluoresces red) and imaged by fluorescence microscopy (magnification 20×). (B) Bar graphs show myocardial (a) and plasma (b) levels of lipid hydroperoxides (LPO) in chronically infected (±SIL) and control mice. (c) Myocardial protein carbonyls by an enzyme-linked immunosorbent assay. Abbreviations as in Figure 1.