Figure 4: Intramyocardial Injection of Anti-CD47 Reduces Acute Infarct Size

(A) Fluorescent microspheres were used to measure area at risk (left) at 24 h after reperfusion. Staining with 2,3,5-triphenyl-tetrazolium chloride was performed to measure infarct size (right). Shown here are representative images of myocardial short axis slices and quantification. There were 10 animals in each group (p = 0.0001). (B) Serum troponin levels after anti-CD47 treatment at 24 h (p = 0.01). (C) CD47 monoclonal antibody injection reduced infarct size as measured by gadolinium enhanced magnetic resonance imaging at 24 h after reperfusion. Example segmentation of the late gadolinium enhancement images. The infarct zones are indicated by the red arrows. The green contours represent the epicardial border, red contours represent the endocardial border, and the yellow contours depict the infarct zones as delineated by automatic thresholding. To the right is quantification of infarct size; n = 7 vs 7. p < 0.05 versus control.



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