Figure 4: Colchicine Treatment Improved Calcium Handling in

(A) Confocal micrographs of mdx cardiac myocytes stained with an α-tubulin antibody to show effective microtubule depolymerization with colchicine treatment. (B) Representative curves of calcium transients. (C–E) Colchicine treatment did not affect baseline calcium, peak calcium, or kinetics of onset of calcium transients, but increased calcium transient decay to 25% of baseline calcium levels (p = 0.03). (F). N = 20 cells to 22 cells from 2 animals to 3 animals per treatment. (G) Representative calcium spark images from isolated mdx cardiac myocytes. Arrows indicate calcium sparks. (H) Quantification of calcium sparks frequency. Colchicine treatment significantly reduced calcium sparks in mdx cardiac myocytes (p = 0.04). N = 17 cells to 20 cells from 3 animals per treatment. ∗p ≤ 0.05 as determined by Student t test. Values are presented as mean ± SEM.