Figure 4: Acute Overexpression of S16D-Hsp20 in Cardiomyocytes Promotes Upregulation of the

(A) Assessment of the following cytokine gene expression levels in Ad.GFP and Ad.S16D cardiomyocytes Ccl2, Ccl3, TNF-α, and IL-6. TGFβ1 and IL-1β were not detectable in either group. Values were normalized to those of the internal control, 18 S, and expressed as fold-changes relative to those of Ad.GFP. (B) Quantification of IL-6 secretion into the conditioned medium from Ad.GFP and Ad.S16D cardiomyocytes was determined by using an ELISA assay. The number of samples (n) per group is indicated on the bar graphs. Ccl2 = C-C motif chemokine ligand 2; Ccl3 = C-C motif chemokine ligand 3; ELISA = enzyme-linked immunosorbent assay; MR16-1 = rat anti-mouse IL-6 receptor monoclonal antibody; TNF = tumor necrosis factor; WT = wild type; other abbreviations as in Figure 3.



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