Figure 3: cMyBP-C-AAb Reactivity in Cardiomyopathy and ACS Patient AAb-Positive Sera Decrease Upon Pre-Adsorption With cMyBP-C

Sera from 3 AAb-positive patient samples from the DCM, HCM, and ACS patient groups for each cMyBP-C fragment and for FL-cMyBP-C were pre-adsorbed with 0, 1, 2, 4 or 8 ng/μl of their respective target protein. Following pre-adsorption, serum AAb reactivity was tested by ELISA for AAbs to (A) C0 to C1f, (B) C0 to C3, (C) C3 to C6, (D) C6 to C8, (E) C8 to C10, and (F) FL-cMyBP-C. A p trend <0.05 was considered statistically significant. Abbreviations as in Figures 1 and 2.