Figure 3: SP16 Induces Rapid Phosphorylation of Akt During Ischemia–Reperfusion

We measured phosphorylation at Ser473 of Akt in myocardial samples during ischemia–reperfusion as a measure of the prosurvival effect of SP16. As compared with vehicle-treated mice, mice receiving SP16 100 μg intraperitoneally at time of reperfusion had a significant increase in pAkt/total Akt ratio, associated with a reduction in the ratio of the proapoptotic to antiapoptotic Bax/Bcl2 ratio and of caspase-3 myocardial tissue activity. i.p. = intraperitoneal; p-Akt = phosphorylated Akt.