Figure 3: Phosphorylation of Total Titin in Nonfailing Donor (Control) and AS Patient Hearts

(A) Representative Western blot (WB) from 1.8% polyacrylamide/1% agarose gel, using antiphosphoserine/-threonine antibodies, as well as Coomassie-stained polyvinylidenfluoride (PVDF) membrane indicating protein load. All bands shown were from the same gel/blot. (B and C) Summary of results of densitometric analysis of titin phosphorylation (N2B isoform), indexed to protein load. Shown are box plots (median [first quartile, third quartile]) with whiskers and individual data points (red circles). (B) Mann-Whitney U test revealed statistically significant differences between control hearts and all AS patients (p = 0.022). (C) Comparison was made between AS subgroups using Kruskal-Wallis test (p > 0.05). Post hoc multiple pairwise comparisons revealed significant differences between control group and cAS, pAS, and ASrEF (*p < 0.05). Abbreviations as in Figure 1.