Figure 3: Granule Proteins and Adhesion Molecules Are Upregulated in LDGs Compared With NDGs at the Gene Level

RNA sequencing analysis was completed on 50,000 LDGs relative to NDGs from psoriasis patients. (A) Differentially expressed genes (n = 1,076) were identified between NDGs and LDGs from psoriasis patients (n = 7). NDGs are the reference, as are upregulated genes in LDGs. (B) The volcano plot shows clear separation between NDGs and LDGs. The upregulated (red) and downregulated (green) transcriptomes are NDGs, as LDGs are the reference sample. (C) The gene ontology biological process analysis highlighted biological processes that were differentially expressed between NDGs and LDGs. Significance was established by false discovery rate (FDR). (D) Granule proteins are upregulated in all patients (n = 7) and when normalized and by (E) FPKM values (n = 7). Data are represented as means ± SEM. Significance was established by the unpaired Mann-Whitney Student’s t-test and set at *p < 0.05. (F) Transmission electron microscopy images of LDGs and NDGs show LDGs had more electron dense granules. AZU1 = azurocidin 1; CTSG = cathepsin G; ELANE = neutrophil elastase; FPKM = fragments per kilobase of transcripts per million; ICAM2 = intercellular adhesion molecule 2; ITGAL = integrin subunit alpha L; ITGAM = integrin subunit alpha M; MPO = myeloperoxidase; P = patient; PRTN3 = proteinase 3; other abbreviations as Figures 1 and 2.