Figure 2: Slice Characteristics

Slices are cut parallel to the myocyte long axis. (A) Slice dimensions, measured wet weight and protein, and estimated myocyte number (based on average volume 26,500 μm3). (B) Beta-myosin heavy chain (β-MyHC) immunohistochemistry in a section tangential to a nonfailing slice shows well-aligned sarcomeres, with some wrinkling by culture day 2, likely caused by contraction. (C) A nonfailing slice cross section stained with wheat germ agglutinin shows doxorubicin (red autofluorescence) in myocyte nuclei in the center of the slice within 1 min of incubation, and clean top and bottom surfaces.



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