Figure 2: Response to Injury Is Significantly Reduced in the TILRR-Knockout Mouse

(A) Immunohistochemistry of a cross-section of a carotid artery in a WT mouse subjected to 4 weeks of ligation. Staining using a nonblocking anti-TILRR antibody followed by a DyLight Fluor-conjugated secondary antibody shows high levels of TILRR expression (red). Scale bar = 150 μm. Trichrome staining of cross-sections of carotid arteries after 4 weeks of ligation in (B) a WT mouse and (C) a TILRR KO mouse. Arrows indicate thickness of the neointima in response to treatment. Scale bar = 150 μm. (D to F) Quantitation of measurements from cross sections as in B and C shows (D, E) a significant reduction in the thickness of the neointima and (F) a decrease in cell number in the adventitia in TILRR KO mice compared with levels in WT mice. Data are expressed as (D, E) area (μm2) and (F) cell number per μm2 and show mean ± SEM. n = 6 WT mice, 7 TILRR KO mice. TILRR KO mice versus WT mice, (D) *p = 0.038, (E) **p = 0.0045, (F) **p = 0.0014. Abbreviations as in Figure 1.