Figure 2: Negative Hyperbolic Relationship Between JPH-2 and Tubulin in Aged

Quantitative Western blot analysis of α- and β-tubulin levels in young (A and B) and aged (C and D) mdx mice. There was no significant difference in tubulin levels in young (2 months to 3 months of age) mice but older (8 months to 10 months of age) mdx mice showed a significant 2.6-fold increase in α- and β-tubulin protein expression. (For α-tubulin comparison, p = 0.0008. For β-tubulin comparison, p = 0.0007.) N = 3 animals to 4 animals per genotype per age point. (E and F) A strong negative hyperbolic relation between JPH-2 and α- and β-tubulin in older mdx cardiac extracts exists (r2 = 0.95 for α-tub and JPH-2 curve-fitting, and r2 = 0.95 for β-tubulin and JPH-2 curve fitting). ∗p ≤ 0.05 as determined using Student t test. Values are presented as mean ± SEM. Abbreviations as in Figure 1.