Figure 2: Flow Cytometry Analysis of iCM Purity

Cardiac troponin T (cTnT), vascular adhesion molecule-1 (VCAM1), and cTnT/VCAM1 double-positive cells demonstrate high purity of induced pluripotent stem cell–derived cardiomyocytes (iCM). VCAM1 was shown to be a potent cell surface marker for robust, efficient, and scalable purification of cardiomyocytes from human embryonic stem cells/human induced pluripotent stem cells (83). FSC = forward-scattered light; LL = lower left; Q5 = human endothelial cells; UL = upper left; UR = upper right; SSC = side-scattered light.