Figure 2: Early Atherosclerosis Development in FF-DM and FF-NDM Swine

Several lesion types were assessed by OCT (A1 to A3) and matched with histology (oil-red-O stain [A4 and A5] and von Kossa stain [A6]). Intimal hyperplasia was defined as a fibrous lesion >150-μm thickness on OCT (A1), a lipid-laden lesion (A2 and A5) as diffusely delineated signal-poor region (A2, arrowheads) and a mixed lesion (A3 and A6) as a lesion containing both lipid (A3, arrowheads) and calcium, which was defined as a sharply delineated signal-poor region ([A3] arrow). In histology, red stain denotes lipid (A5), and closed bars denote calcium (A6) deposits. (B) The prevalence and burden (C and D) of disease increased from 9 to 15 months, without any significant differences between FF-DM and FF-NDM. *Guidewire artifact. Abbreviations as in Figure 1.