Figure 2: BAG3 Overexpression Improves Myocyte Contractility Post-MI

Myocytes were isolated from the left ventricles and septum of the 3 groups of mice (n = 3 mice each) used in Figure 1, with care being taken to excise the infarct scar in MI-GFP and -BAG3 hearts. In Sham-GFP hearts, the area corresponding to the infarct scar in MI hearts was excised before myocyte isolation. Representative contraction traces obtained in freshly isolated myocytes (2 Hz, 37°C, 1.8 mmol/l [Ca2+]o) (please see the Methods section) are shown at baseline (A) and after 1 μmol/l of isoproterenol (Iso) (B). Contraction amplitudes (C), maximal shortening (D), and re-lengthening velocities (E) are shown for Sham-GFP, MI-GFP, and MI-BAG3 myocytes, both at baseline and in the presence of isoproterenol. There are 17 Sham-GFP, 18 MI-GFP, and 25 MI-BAG3 myocytes at baseline and 21 Sham-GFP, 18 MI-GFP, and 17 MI-BAG3 myocytes after isoproterenol. Differences between groups are analyzed with 2-way analysis of variance are given in the Results section. RCL = resting call length; other abbreviations as in Figure 1.