Figure 1: PP2Ce is an ER Membrane-Targeted Protein Phosphatase and Detected in Adult Mouse Hearts

(A) Schematic description of protein phosphatase 2C on endoplasmic reticulum (ER) membrane (PP2Ce) protein with signal peptide sequence (aa20-40), transmembrane domain, and conserved PP2C phosphatase activity domain highlighted. (B) Northern-blot analysis of PP2Ce messenger ribonucleic acid (mRNA) expression in different tissues from adult mice with significant expression detected in in the brain, heart, and diaphragm. (C) Immunohistochemistry results displayed PP2Ce (green) was colocalized with sarcolemma-endoplasmic reticulum calcium ATPase (SERCA) 2a (red) in adult rabbit ventricular cardiomyocytes as supported in merged image and line-scanning intensity analysis. (D) Relative expression of PP2Ce mRNA detected by quantitative real-time polymerase chain reaction in heart samples from ischemic cardiomyopathy (ICM) (n = 3) and dilated cardiomyopathy (DCM) (n = 3) compared with non-heart failure hearts (normal, n = 3). *p < 0.05 versus normal.



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