Figure 1: Functional Perfusion Study Design in a Rat Hindlimb Ischemia Model

(A) In vivo study timeline. (B) Hindlimb perfusion measured with laser speckle contrast analysis (LASCA). Image is post-surgery with a significant decrease in perfusion of the ischemic (right) limb compared to the healthy (left) limb. (C) Example perfusion measurement for a single ischemic animal. Healthy limb (blue line), the ischemic limb (red line), and total body (black line) are plotted over time in arbitrary units or perfusion units. Perfusion kinetics ratio was calculated by dividing the slope of the linear region of the healthy limb (blue dotted line) by the slope of the linear region of the ischemic limb (red dotted line). (D) Pre-surgery and immediately post-surgery readings for each treatment group. Histo = histology; Inj = injection; IS = ischemia surgery; PM = perfusion measurement.