Figure 1: Experimental Protocol

(A) Following baseline data collection, the left anterior descending coronary artery was occluded distal to the second diagonal branch with an inflatable angioplasty balloon for 10 min, followed by reperfusion for 1 h (n = 5) or 24 h (n = 5). Post-mortem TUNEL analysis was performed on myocardial tissue sections collected from ischemic and non-ischemic regions of the left ventricle. (B) Example fluoroscopic images illustrating the percutaneous coronary occlusion procedure. LAD = left anterior descending coronary artery; TTC = triphenyltetrazolium chloride; TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.