Figure 1: ELISAs to Measure ATX-Lp(a) and ATX-apoB

Methodology of novel chemiluminescent enzyme-linked immunoadsorbent assay (ELISA) to measure autotaxin (ATX)–apolipoprotein(a) (apo[a]) and ATX–apolipoprotein B (apoB) complexes. ApoB-100 and lipoprotein(a) (Lp[a]) lipoproteins were captured from plasma with specific antibodies bound to microtiter well plates. Conditions were established so that the added plasma contains saturating amounts of lipoproteins to be captured. The content of ATX mass on each lipoprotein was then detected with a goat anti-human ATX antibody.