Central Illustration: Phenotypic Screens in Zebrafish

2019-06-11T07:03:23Z (GMT) by Aaron Kithcart Calum A. MacRae
The core concept of the phenotypic screen is illustrated. Using a genetically modified fish, it is possible to recapitulate much, if not all, of the fundamental biology of the disease as well as the molecular and cellular homeostatic responses to the primary defect. Because zebrafish offspring can survive in multiwell plates, if high-throughput phenotypes can be developed that represent the disease endpoints, a chemical suppressor screen can be undertaken to identify tool compounds or drug leads. Such screens have been successfully undertaken to prioritize hundreds or lead compounds or to screen in an unbiased manner hundreds of thousands of small molecules. Importantly, toxicity can also be addressed in parallel during the same screens.