Figure 8: RBCs From Patients With Type 2 Diabetes Impair Post-Ischemic Cardiac Function of Isolated Rat Hearts via an Arginase and NOS-Dependent Mechanism

Rat hearts were given (A) RBCs from either HS (n = 9) or patients with DM (n = 13), (B) RBCs from patients with DM and incubated with vehicle (n = 13), nor-NOHA (1 mmol/l, n = 12), or L-NAME (0.1 mmol/l) + nor-NOHA (1 mmol/l, n = 6), (C) RBCs from DM and incubated with vehicle (n = 13) or L-NAME (0.1 mmol/l, n = 5), (D) RBCs from HS and incubated with vehicle (n = 9) or L-NAME (0.1 mmol/l, n = 9), (E) RBCs from HS and incubated with vehicle (n = 6) or 1400W (0.1 mmol/l, n = 5) or RBCs from DM and incubated with vehicle (n = 5) or 1400W (n = 5), or (F) RBCs from HS incubated with vehicle (n = 9), nor-NOHA (1 mmol/l, n = 9), or L-NAME (0.1 mmol/l) + nor-NOHA (1 mmol/l, n = 9). Significant differences between treatments were analyzed using 2-way analysis of variance including all time points; *p < 0.05 and ***p < 0.001. Abbreviations as in Figures 1, 2, 3, and 4 and 7.