Figure 8: Increased Glycolysis in SUR2/

(A) Phosphoenolpyruvate was significantly increased in Cre+ hearts at baseline as measured using metabolomic profiling (p = 0.01). (B) Phosphocreatine levels were increased in MCM Cre+ hearts (p = 0.05). (C) The ratio of glycogen over protein was not significantly different between MCM Cre– and MCM Cre+ hearts. (D) Whole hearts were equilibrated on a Langendorff apparatus for 5 min and subjected to 13C-glucose uptake for 20 min followed by 5 min with unlabeled 12C-glucose and freezing for analysis. MCM Cre+ hearts had a significant increase in unlabeled carbon (13C-0) D-glyceraldyhade-3-phosphate and 3-phosphoglycerate, while 3-phosphoglycerate also had a significant reduction in (13C-3) (n = 3 MCM Cre–, n = 4 MCM Cre+ hearts). *p = 0.05. (E) Steps in the glycolysis pathway. Red asterisks indicate substrates that is statistically different between MCM Cre– and MCM Cre+ hearts. X indicates substrates that were not measured. NS indicates substrates that were measured and were not statistically different between MCM Cre– and MCM Cre+ hearts. AU = arbitrary units; MDV = mass distribution vector; other abbreviations as in Figures 1 and 2.

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