Figure 7: Granulation Tissue Response and Immune Cell Accumulation in the Adventitia of Silk Grafts

(A) Representative micrograph images of hematoxylin and eosin (H&E) staining of the adventitia surrounding the silk grafts at all time points. Scale bar = 100 μm. (B) Representative fluorescent micrograph images of CD68 immunostaining at all time points. Scale bar = 100 μm. (C) Quantification of granulation tissue area, represented as a percentage of total lumen size for each graft. N = 4 animals per time point with 3 images analyzed per region per graft. (D) Quantification of positive CD68 staining, indicating macrophage presence, in the granulation tissue area, represented as a percentage of total granulation tissue for each section. N = 4 animals per time point with 3 images analyzed per region per graft. CD68 staining is red, and 4′,6-diamidino-2-phenylindole staining is blue. Scale bar = 100 μm. The red dashed lines indicate the average granulation tissue thickness values (C) and average CD68 staining (D) for each time point. Statistical analysis was performed on these values. D → P indicates selected equidistant regions within the graft, from distal to proximal.

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