Figure 7: Effects of the NPR-C Activator cANF on Ang II-Mediated Alterations in AP Morphology and Hyperpolarization-Activated Current (I

(A) Representative spontaneous AP recordings obtained in wild-type mice treated with saline, Ang II, or Ang II plus cANF (0.07 or 0.14 mg/kg/day). (B) (C) Summary of cycle length (B) and DD slope (C) illustrate the effects of cANF co-treatment on Ang II-mediated changes in SAN AP morphology. (B) *p < 0.001 versus saline; #p = 0.006 versus saline; +p = 0.046 versus Ang II; $p = 0.008 versus Ang II. (C) *p < 0.001 versus saline; +p = 0.014 versus Ang II; $p < 0.001 versus Ang II; p = 0.024 versus Ang II plus cANF (0.07). Data were analyzed by 1-way analysis of variance with Tukey post hoc test; n = 16 saline, 11 Ang II, 13 Ang II plus cANF (0.07), and 10 Ang II plus cANF (0.14) for AP data. See Supplemental Table S4 for additional AP parameters. (D) Representative If recordings from wild-type mice treated with saline, Ang II, or Ang II plus cANF (0.07 or 0.14 mg/kg/day). (E) (F) Summary IV curves for the effects of Ang II and cANF (0.07 and 0.14 mg/kg/day) on If. (G) (H) If activation curves illustrating the effects of Ang II and cANF (0.07 and 0.14 mg/kg/day) in SAN myocytes. (E) *p < 0.001 versus saline; +p = 0.002 versus Ang II. (F) *p < 0.001 versus saline; +p < 0.001 versus Ang II. Data were analyzed by 2-way repeated-measures analysis of variance with Tukey post hoc test; n = 17 cells for saline, 13 cells for Ang II, 14 cells for Ang II+cANF (0.07), and 13 cells for Ang II plus cANF (0.14). See Supplemental Figure S10 for additional If kinetic analysis. Abbreviations as in Figures 1, 2, 3, and 5.