Figure 7: Effect of PP2Ce on Calcium Homeostasis in Intact Mouse Heart

(A) Schematic drawing of PP2Ce-transgenic mouse with tissue-specific expression of PP2Ce in heart. (B) PP2Ce protein expression in mouse hearts from 4 genotypes carrying either none, single, or double transgenes of αMHC-tTA expressing tet-off suppressor (tTA) and αHMC-TetO-PP2Ce (iPP2Ce). PP2Ce expression was detected only from double transgenic hearts in the absence of tetracycline. PC is the positive control from NRVM-expressing adv-PP2Ce. (C) Representative immunoblot analysis. (D) Quantification of key proteins involved in calcium homeostasis regulation in control and PP2Ce transgenic (TG) hearts. Number of replicates is labeled in each column. (E) Calcium transient peak amplitudes (F/F0), time to peak and decay measured from isolated cardiomyocytes of control (n = 8) and PP2Ce TG (n = 8) hearts. *p < 0.05; **p < 0.01. Abbreviations as in Figures 2, 3, and 4.

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