Figure 7: Cardiac Myocyte Volume

Cardiac myocytes were isolated 9.0 ± 0.2 weeks after intravenous delivery of saline, AAV8.UCn2 (1.9 × 1013 gc/kg) or AAV8.UCn3 (1.9 × 1013 gc/kg). Viable isolated cardiac myocytes were fixed in 10% formalin, stained with eosin and their size was measured using ImageJ software. (A) Each symbol represents the mean volume of 120 ± 11 cardiac myocytes measured per mouse. Measures were made blinded to group identity. (B) Representative isolated cardiac myocytes, indicating cardiac myocyte length (red) and width (green). There was an overall group difference in cardiac myocyte volume (p < 0.02, Kruskal-Wallis) due to a larger volume in cardiac myocytes from control mice. Between-group comparisons are from the Dunn multiple comparison test. Abbreviations as in Figures 1, 2, and 6.