Figure 7: Administration of IGF-1/HGF and UPy IGF-1/HGF Stimulates Moderate Skeletal Muscle Regeneration

(A) HVG staining of uninjured, CTX:IGF-1/HGF–, and CTX:UPy IGF-1/HGF–treated muscle, scale bar = 100 μm. (B) Immunohistochemical staining shows a number of BrdUpos centralized nuclei in both CTX:IGF-1/HGF– and CTX:UPy IGF-1/HGF–treated muscle compared with uninjured muscle, scale bar = 50 μm. (C) Quantification of the ratio of skeletal muscle to connective tissue CSA in uninjured, CTX:IGF-1/HGF–, and CTX:UPy IGF-1/HGF–treated muscle determined from 5 fields of view per muscle, n = 5 animals per group. Data are mean ± SD, *p < 0.01 versus uninjured CTRL. (D) BrdUpos centralized nuclei per total myofibers, determined from five fields of view per muscle, n = 5 animals per group. Data are mean ± SD, *p < 0.001 versus uninjured CTRL. (E) Average myofiber diameter of regenerating (BrdUpos centralized nuclei) myofibers determined from 5 fields of view per muscle, n = 5 animals per group. Data are mean ± SD, *p < 0.0001 versus uninjured CTRL. Abbreviations as in Figure 1.