Figure 6: The pPIC Secretome Promotes a Regenerative Response in Vitro

(A) Quantification of pPIC BrdU 24-h proliferation assay in standard growth media (GM), 24-h pPIC conditioned media (CM), unconditioned media (UM), or heat-inactivated conditioned media (ICM). Total BrdUpos cells per total nuclei, determined from 5 fields of view per group. Data are mean ± SD, *p < 0.0001 versus all conditions. (B) Quantification of human myoblasts BrdU 24-h proliferation assay in GM, CM, UM, or ICM. Total BrdUpos cells per total nuclei, determined from 5 fields of view per group. Data are mean ± SD, *p < 0.0001 versus UM and ICM. (C) Quantification of BrdU 24-h proliferation assay in medium supplemented with IGF-1, HGF, TGF-β1, or NRG-1 compared with control. Total BrdUpos cells per total nuclei, determined from 5 fields of view per group. Data are mean ± SD, *p < 0.0001 versus control and TGF-β1. (D) Representative micrographs of pPIC immunocytochemical staining for MHC after 5 days of myogenic differentiation in the presence of IGF-1, HGF, NRG-1, or TGF-β1 compared with control. (E) Quantification of pPIC fusion index after 5 days of myogenic differentiation in the presence of IGF-1, HGF, TGF-β1, or NRG-1 compared with control. Data are mean ± SD, *p < 0.0001 versus control for >3 nuclei. (F and G) Endothelial network formation quantified as capillary area (F) and total length of capillaries (G), after human umbilical vein endothelial cells were exposed to endothelial GM, CM, UM, and ICM. Data are mean ± SD, *p < 0.05 versus ICM and GM. MHC = myosin heavy chain; other abbreviations as in Figure 1.