Figure 6: Short-Term Histological Assessment of Skeletal Muscle Progenitor Cells

(A) Representative image of Pax-7+ (red) and Hoechst 33342 (blue) costaining in skeletal muscle matrix (SKM)–injected gracilis muscle. Scale bar is 50 μm and Pax-7+ nuclei are indicated by white arrows. There were significantly more Pax-7+ cells as a percentage of total nuclei in SKM (n = 6) versus saline (n = 6) injected muscles at (B) day 3, but this returned to quiescent levels by (C) day 10. *p = 0.0096. Data from the healthy limb is shown as a reference.