Figure 6: Paracrine Effect of AE-Secreted ET-1 on CM Ca

Bar graphs showing (A) endothelin (ET)-1 mRNA levels in stretched AE cells (n = 3), and (B) mean TRPC-6 expression in the cell membrane of control and stretched AE cells (n = 3). (C) Ca2+ transient traces in CMs under basal conditions (control cells) and in cells pre-conditioned with media from nonstretched and stretched AE cells with or without addition of 1 nM of ET-1 peptide. (D) Mean Ca2+ transient amplitude and frequency in CM bathed in Tyrode’s solution (control cells) or pre-conditioned with media from nonstretched and stretched AE cells, with or without addition of 1 nM of ET-1 peptide. (E) Ca2+ transient traces in CMs pre-treated with BQ-123 and pre-conditioned with media from non-stretched and 24-h stretched AE cells. All values are mean ± SEM. *p < 0.05 versus control cells; p < 0.025 versus nonstretched; §p < 0.001 versus 4-h stretch. BQ-123, ET-1 receptor (ETA) inhibitor; other abbreviations in Figures 1, 2, and 5.