Figure 6: None

(A) Expression of CARMEN7, MIR-143 MIR-145, ELK1, and KLF4 transcripts in proliferating CPCs (Expansion [Expansion]; black) and in differentiated (Differentiation) adult CPCs without prior DLL1 stimulation and cultured in the absence of DAPT (None No DAPT). Cells were transfected with either scrambled GapmeRs (white), GapmeRs directed against all CARMEN isoforms (light gray), or GapmeRs directed specifically against CARMEN7 (dark gray). Data represent mean ± SEM; *p < 0.05 as compared with proliferating CPCs; ‡p < 0.05 as compared with differentiating CPCs (white) transfected with scrambled GapmeRs (n = 3). (B) Expression of sarcomeric proteins (MYH6, MYH7, MYH11) in proliferating CPCs (Expansion; black) and in differentiated adult CPCs exposed or not to DLL1 in the absence or presence of DAPT. Cells were transfected with either scrambled GapmeRs (white), GapmeRs directed against all CARMEN isoforms (light gray), or GapmeRs directed specifically against CARMEN7 (dark gray). Data represent mean ± SEM; *p < 0.05 as compared with proliferating CPCs; ‡p < 0.05 as compared with differentiating CPCs (white) transfected with scrambled GapmeRs (n = 3). (C) Analysis of SM-MHC, α-ACTININ, TNNI, and MLC2V expression by immunostaining in differentiated CPCs exposed to DLL1 in the presence of DAPT, transfected with either scrambled GapmeRs (white), GapmeRs directed against all CARMEN isoforms (light gray), or GapmeRs directed specifically against CARMEN7 (dark gray). Scale bar: 25 μm. Quantification of the numbers of α-ACTININPOS, TNNIPOS, or MLC2VPOS cardiomyocytes and sm-MHCPOS smooth muscle cells. Data represent mean ± SEM; *p < 0.05 as compared with proliferating CPCs; ‡p < 0.05 as compared with differentiating CPCs (white) transfected with scrambled GapmeRs (minimum 1,500 cells in 20 different fields at a magnification of 40× per quantification).