Figure 6: Histologic Assessment of Neointima Formation and SMC Phenotype in Silk Grafts

(A) Representative micrograph images of hematoxylin and eosin (H&E) staining of silk grafts at all time points. Scale bar = 200 μm. (B) Representative fluorescent micrograph image of proliferating cell nuclear antigen (PCNA)/smooth muscle cell actin (SMC-α) double stain at all explantation time points. Scale bar = 200 μm. (C) Quantification of neointima, represented as a percentage of total lumen size for each graft. N = 4 animals per time point with 3 images analyzed per region per graft. (D) Quantification of positive PCNA (red) and SMC-α (green) staining, represented as a percentage of the total positive staining in each graft section. N = 4 animals per time point with 3 images analyzed per region per graft. The dashed lines indicate the average neointima values (C) and average PCNA and SMC-α actin staining (D) for each time point. Statistical analysis was performed on these values. D → P indicates selected equidistant regions within the graft, from distal to proximal.

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