Figure 6: Effects of SP16 on Cardiomyocyte Apoptosis and Myocardial Inflammation and Fibrosis
2019-06-11T07:05:53Z (GMT) by
We measured the percentage of (A) apoptotic cardiomyocytes by detecting nuclei showing in situ end labeling of deoxyribonucleic acid fragmentation (terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL]) in the peri-infarct border zone, (B) the number of leukocytes (positive for cluster of differentiation 45) per high-power field (HPF) ×40, and (C) the extent of the fibrotic infarct scar (at Masson trichrome stain) in the mouse hearts 7 days after 30 min of ischemia. Treatment with SP16 led to a significant reduction in infarct scar size and a reduction in cardiomyocyte apoptosis and myocardial inflammation in the peri-infarct border zone. n = 4 mice/group. LV = left ventricle.