Figure 6: Combined Effects of Valsartan With LBQ657 in In Vitro Assay Using Peritoneal Macrophages

(A) Gelatin zymography on the supernatants of peritoneal macrophages stimulated with LPS (10 ng/ml) for 48 h. (B) Semi-quantitative analysis of gelatinolytic activity showed significantly lower activity in VAL + LBQ compared to the VAL-alone and LBQ-alone groups. n = 6 per group. Quantification of (C) IL-1β, and (D) IL-6 concentrations in the supernatants of peritoneal macrophages stimulated with LPS (10 ng/ml) for 48 h. n = 6 per group. (E to G) Real-time reverse transcriptase PCR result for (E) MMP-9, (F) IL-1β, and (G) IL-6 mRNA level in peritoneal macrophages stimulated with LPS (10 ng/ml) for 48 h. The mRNA level was normalized by the level of endogenous control beta-2-microglobulin RNA. n = 8 per group. Results were mean ± SD. Ctrl = control; LBQ = LBQ657; LPS = lipopolysaccharide; VAL = valsartan; other abbreviations as in Figure 4.