Figure 5: TILRR Antibody Administration Reduces Inflammatory Responses in the Lung

(A) Macrophages in lung sections from ApoE–/– mice fed a high-fat diet (left, Mac-3 green) and inducible bronchus-associated lymphoid tissue (iBALT) structures (inset) show high TILRR expression (right, red). Scale bar = 150 μm. (B) Quantitation of macrophages from images as in A. Data are expressed as number of macrophages per 0.15 mm2 and show mean ± SEM. n = 9 control mice (black bar), 7 TILRR antibody–injected mice (light gray bar). Levels in antibody-injected mice versus levels in control mice, ***p = 0.001. (C) Trichrome staining of lung sections from ApoE–/– mice from control (left) and mice injected with the anti-TILRR peptide antibody (right). Scale bar =150 μm. (D) Fibrous grade, (Ralf-Harto Hübner scale) in control (black bar) and TILRR antibody–injected (light gray bar) mice. Data show mean ± SEM, n = 9 control, n = 7 TILRR antibody–injected mice. Levels in antibody-injected mice versus levels in control mice, *p = 0.0472. (E) Granulomas (arrow) and iBALT (arrowhead) in lung tissue from an ApoE–/– mouse. Scale bar =150 μm. (F, G) Quantitation of sections as in panel E shows (F) levels of granulomas and (G) iBALT structures in control mice (black bar) and TILRR antibody–injected mice (light gray bar). Data are expressed as number of structures per cross-sectional area, as indicated. n 9 control mice, 7 antibody-injected mice. Levels in antibody-injected mice versus levels in control mice, (F) *p = 0.0263; (G) *p = 0.0452. (H) Lung section from a control animal with muscularized vessels with TILRR expression in surrounding tissue (top; TILRR, red; SMC α-actin, green; DAPI, blue). Trichrome staining (bottom). Scale bar = 75 μm. (I) Muscularized vessels in a control (top image) and in a TILRR antibody–treated mouse (bottom image). Scale bar = 75 μm. (J) Morphometry of medial area in vessels from control (black bar) and antibody-injected mice (light gray bar). Values are mean ± SEM. n = 7 control, n = 7 TILRR antibody–injected mice. Levels in antibody-injected mice versus levels in control mice, *p = 0.0152. Abbreviations as in Figures 1 and 4.