Figure 5: Mechanical Integrity of Sterile Valve Cusps

(A) Stiffness of the sterile valve leaflets was compared with that of non-sterile decellularized valve leaflet. Stiffness of native valve leaflet (∗p < 0.0001) and LHP treated leaflet (∗p = 0.002) were significantly different from decellularized leaflet by 1-way ANOVA. (B) Ultimate tensile strength (UTS) of the sterile valve leaflets was compared with that of non-sterile decellularized valve leaflet. UTS of native valve leaflet (∗p < 0.0001), ETPA treated (∗p < 0.0001), LHP treated (∗p = 0.0001), and scCO2 treated leaflets (∗p < 0.0001) were significantly different from decellularized leaflet by 1-way ANOVA. ETPA = 96% ethanol with 2% peracetic acid; other abbreviations as in Figure 3.