Figure 5: Intramyocardial Injection of Anti-CD47 Improves Systolic Function and Reduces Fibrosis

(A) Assessment of heart function after ischemia-reperfusion in CD47-antagonized mice at indicated days (d) after I/R. Images are M-mode tracings. Control average heart rate = 474 and anti-CD47 heart rate = 507. (B) Echocardiography analysis of percent ejection fraction. Day 7, p = 0.03; day 21, p = 0.03; day 28, p = 0.03 (comparisons are between ISO and anti-CD47 at each indicated day). (C) Left ventricular (LV) systolic and diastolic dimensions. The LV anterior wall diastolic thickness (LVAWDd) (mm) on day 28 from IsoIgG and α-CD47 antibody-injected mice (*p = 0.04). (D) Hearts harvested 21 to 28 days after I/R for staining with Masson’s trichrome blue. ISO, isotype control. Scale bar, 1.5 mm. *p = 0.04 relative to control. The number of animals in each group = 10 versus 10. Abbreviations as in Figure 1.

Categories

License

Elsevier user license