Figure 5: ISO-Mediated Regulation of PP2Ce Protein in Cardiomyocytes

(A) PP2Ce and PP2Cα expression in NRVM following ISO treatment. (B) PP2Ce expression in NRVM treated with ISO (1 μmol/l) with or without cotreatment of proteasome inhibitor MG132 (10 μmol/l) or lysosomal inhibitor BAF (50 nmol/l) as indicated. (C) Quantification of PP2Ce expression in NRVM treated with ISO with or without additional treatment of cycloheximide (CHX), MG132, or BAF as indicated. (D) PP2Ce expression in NRVM-treated ISO or forskolin (FSK) at 0.1, 1, and 10 μmol/l concentrations as indicated. (E) PP2Ce expression in NRVM treated with FSK (1 μmol/l) with or without PKA inhibitor H89 (1 μmol/l) as indicated. n indicates the number of total experimental replicates. *p < 0.05; **p < 0.01. Abbreviations as in Figures 2, 3, and 4.

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