Figure 5: Effects of SP16 on LV Systolic Function

We measured left ventricular (LV) systolic function with SP16 at rest and after challenge with isoproterenol to evaluate also contractile reserve. SP16 had no significant effect on LV fractional shortening (LVFS) (A) at rest or (B) after isoproterenol challenge. These data show that, as tested, SP16 has no appreciable inotropic activity in vivo. We also measured LVFS at 7 days after acute myocardial infarction (AMI) in mice. (C) Mice treated with SP16 at reperfusion had significantly greater LVFS than did vehicle-treated mice. (D) This was associated with a significantly smaller area of wall motion abnormality, a surrogate for infarct size. These data show that the early cardioprotective effects of SP16 are maintained at 7 days after surgery. n = 5 to 8 mice/group. d = days.